You can run any 12AX7 in any position (V1-V5) without doing anything other than unplugging and plugging a new one in it's socket.
Powertubes are a different story.
If you ask any non-mesa owner with a fixed bias amp we will tell you that you absolutely MUST bias your amp when swapping powertubes.
A bit different with Mesas though. I'll try to explain this in 1million words or less.
You can't just replace a worn timing chain or belt in your (old) car without adjusting the ignition timing. It'll probably run but not at it's best. Performance wont be great....probably wont at least.
Now will your wife notice that the plugs are firing a little early or late? Probably not. Unless it's backfiring like crazy or running-on when she kills the ignition.
Well, Mesa decided, to continue to use the timing belt analogy...hey we will set-up all our cars to run in the safest/most reliable range. Then we will sell MESA branded replacement timing belts that when you install one, it will be the same dimensions and specs as the original stock belt. SO, when our customers install a new timing belt they wont have to re-adjust the timing. It will just fall back into acceptable timing range if everything else in the engine is left alone.
Their ads would say..."With Mesa cars you will never again have to adjust timing. Having issues? Simply head to your authorized Mesa dealership and pick-up a new timing belt and poop her in! No need for adjustments ever again. You can throw your bias probe...I mean timing gun into the TRASH!".
Well that's all fine and good...until you want to race that Mesa car or just change it's performance or if you want the timing advanced a bit or want to add a CAI or headers or a new chip or burn lower octane fuel..or higher octane...or if you want to change compression...you will NEED to adjust the timing to squeeze every last drop of performance out of it.
OK so Mesa does a similar thing with their power sections.
They sell their amps with non-adjustable fixed bias so you CAN'T adjust the timing...I mean bias. They say that as long as you continue to buy MESA branded tubes (can you say held hostage?), your amp will operate within ACCEPTABLE parameters and will last a long time..the tubes will I mean.
Some of us though, especially fixed bias EL34 amp players who like some power section crunch and overdrive, want to be able to run ANY powertubes. Mesas, non-Mesas, old, new, Used, NOS....
Now, you CAN go to an online tube dealer and tell them you want JJs or Winged-C- 34s etc...for your mesa, they will sell you a set that will work "acceptably" in your Mesa without the need to adjust the amp's bias.
IF you want to run vintage or un-matched or even new production tubes that just aren't "Mesa rated"...while you might get lucky and stumble upon a set that coincidently falls within Mesa range, odds are that you wont.
The only answer, if you want to be able to run ANY powertubes properly and to enjoy their best performance, you will need to adjust the bias. No worries...all that requires is to clip one stock bias resistor and replace it with one of a different value in series with an adjustable trimpot. Cheap and easy and WAY worth it if you want to be able to adjust your timing!