Not sure about what tubes, but 1st try putting a patch cord in the loop. If that doesn't work, try swapping out the 1st 3 12Ax7's one at a time.(V4 is the rythm channel).
Yup , Fxrs is right.
I have the same experience , if you don't use the fx loop turn the mix pot
to 0% or put a patch cord in the loop will fix the issue.
That's because there's no loading in the loop.